Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia.

نویسندگان

  • A S Al-Wadai
  • M R Al-Othman
  • M A Mahmoud
  • A R M Abd El-Aziz
چکیده

Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus were screened for their ability to produce aflatoxins using HPLC. Thirteen isolates produced aflatoxins ranging from 0.5 to 2.6 µg/kg. Inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD) molecular markers were used, with the aim of genetically characterizing strains of A. flavus to discriminate between aflatoxigenic and non-aflatoxigenic isolates. RAPD and ISSR analysis revealed a high level of genetic diversity in the A. flavus population, useful for genetic characterization. Clustering based on RAPD and ISSR dendograms was unrelated to geographic origin. RAPD and ISSR markers were not suitable to discriminate aflatoxigenic and non-aflatoxigenic isolates, but ISSR primers were better compared to RAPD.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular characterization of mycobiota and aflatoxin contamination of retail wheat flours from Jeddah markets.

The mycological profile of the retail wheat flour selling in different markets at Jeddah (Kingdom of Saudi Arabia) was studied. The most common genera were Aspergillus (isolated from 70% of the tested samples), Penicillium (30%), Eurotium (14%), and in a lesser extent Fusarium (20%) and Alternaria (18%). Twenty-nine strains of Aspergillus flavus were screened for their ability to produce aflato...

متن کامل

Characterization of aflatoxigenic Aspergillus flavus and A. parasiticus strain isolates from animal feedstuffs in northeastern Iran

Aflatoxins are secondary toxic metabolites produced by some Aspergillus spp. particularly, Aspergillus flavus and A. parasiticus that contaminate food and feed. The objective of this study was to evaluate the contamination of feedstuffs with Aspergillus spp. and detect genes involved in the aflatoxin biosynthesis pathway of A. flavus and A. parasiticus isolates. A total of 110 cow feed samples ...

متن کامل

Evaluation of Contamination of Aspergillus flavus and Aflatoxin Production in Pistachio Cultivars and Investigation of a Chemical Controlling Method

In order to study the contamination of Aspergillus flavus and aflatoxin production in pistachio cultivars in the Semnan province, eight cultivars of pistachio were collected from major pistachio growing areas. Using the serial dilution method, ground pistachio kernels were inoculated on plates containing AFPA medium and incubated at 28° C. This experiment was performed using three replications ...

متن کامل

Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains.

Twelve species from six fungal genera were found to be associated with corn (Zea mays L.) grain samples collected from three main regions of Saudi Arabia. The average frequencies of the most common genera were Aspergillus (11.4%), Fusarium (9.5%), Penicillium (5.1%), and Alternaria (5.8%). Fifteen isolates of Aspergillus flavus were screened by HPLC for their ability to produce aflatoxins (AF)....

متن کامل

Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi

  In the present study, mycobiota and natural occurrence of aflatoxin B1 (AFB1) in pellet feed and feed ingredients used in a feed manufacturing plant for rainbow trout nutrition was investigated. The samples were cultured on the standard isolation media for 2 weeks at 28 ºC. Identification of fungal isolates was implemented based on the macro- and microscopic morphological criteria. AFB1 was d...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Genetics and molecular research : GMR

دوره 12 3  شماره 

صفحات  -

تاریخ انتشار 2013